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bgi stomics  (Spatial Transcriptomics Inc)

 
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    Spatial Transcriptomics Inc bgi stomics
    Bgi Stomics, supplied by Spatial Transcriptomics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Schematic diagram illustrating the sub-anatomical compartments within the embryonic telencephalon, including the ventricular zone (VZ), sub-ventricular zone (SVZ), and mantle zone (MZ). (B) Expression of sub-anatomical markers across sections from E12.5 (left), E13.5 2x coronal, 1x sagittal (middle), E14.5 (right). From left to right column: VZ markers, SVZ markers, and MZ markers. From top to bottom row: telencephalon markers (VZ: Fabp7 , SVZ: St18 , MZ: Dcx ); dorsal telencephalon markers (VZ: Pax6 , SVZ: Eomes , MZ: Tbr1 ), and ventral telencephalon markers (VZ/SVZ: Ascl1, Olig2 , MZ: Nkx2-1 ). (C) Cell type classification following unsupervised clustering and manual annotation. WT sections (top row) and Dlx1/Dlx2 -/- sections (bottom row) for E12.5 (left), E13.5 (2x coronal, 1x sagittal) (middle) and E14.5 (right). v/dNP: ventral/dorsal neural progenitors, v/dIP: ventral/dorsal intermediate progenitors, ThalNeur: thalamic neuron, HypothalNeur: hypothalamic neuron. (D) UMAP plots showing clustering and cell type classification for WT and Dlx1/Dlx2 -/- sections. Legend as shown in (C). (E) Dot plot showing the mean expression of cluster markers in cells in each cluster from (C). Dot sizes denote the fraction of cells expressing the corresponding markers. (F) Bar plot showing the percentage of each cell type in WT and Dlx1/Dlx2 -/- E12.5-E14.5 spatial <t>transcriptomics</t> dataset, summarised based on age and genotype. Legend in (C). (G) Volcano plot showing the differential expression analyses comparing VZ and SVZ of WT vs Dlx1/Dlx2 -/- GE. Thresholds for differentially expressed genes were set at FDR<0.05 and fold change > 1 or < -1. (H) Gene ontology analysis results of all differentially expressed genes in VZ and SVZ of Dlx1/Dlx2 -/- GE.
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    (A) Schematic diagram illustrating the sub-anatomical compartments within the embryonic telencephalon, including the ventricular zone (VZ), sub-ventricular zone (SVZ), and mantle zone (MZ). (B) Expression of sub-anatomical markers across sections from E12.5 (left), E13.5 2x coronal, 1x sagittal (middle), E14.5 (right). From left to right column: VZ markers, SVZ markers, and MZ markers. From top to bottom row: telencephalon markers (VZ: Fabp7 , SVZ: St18 , MZ: Dcx ); dorsal telencephalon markers (VZ: Pax6 , SVZ: Eomes , MZ: Tbr1 ), and ventral telencephalon markers (VZ/SVZ: Ascl1, Olig2 , MZ: Nkx2-1 ). (C) Cell type classification following unsupervised clustering and manual annotation. WT sections (top row) and Dlx1/Dlx2 -/- sections (bottom row) for E12.5 (left), E13.5 (2x coronal, 1x sagittal) (middle) and E14.5 (right). v/dNP: ventral/dorsal neural progenitors, v/dIP: ventral/dorsal intermediate progenitors, ThalNeur: thalamic neuron, HypothalNeur: hypothalamic neuron. (D) UMAP plots showing clustering and cell type classification for WT and Dlx1/Dlx2 -/- sections. Legend as shown in (C). (E) Dot plot showing the mean expression of cluster markers in cells in each cluster from (C). Dot sizes denote the fraction of cells expressing the corresponding markers. (F) Bar plot showing the percentage of each cell type in WT and Dlx1/Dlx2 -/- E12.5-E14.5 spatial <t>transcriptomics</t> dataset, summarised based on age and genotype. Legend in (C). (G) Volcano plot showing the differential expression analyses comparing VZ and SVZ of WT vs Dlx1/Dlx2 -/- GE. Thresholds for differentially expressed genes were set at FDR<0.05 and fold change > 1 or < -1. (H) Gene ontology analysis results of all differentially expressed genes in VZ and SVZ of Dlx1/Dlx2 -/- GE.
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    (A) Schematic diagram illustrating the sub-anatomical compartments within the embryonic telencephalon, including the ventricular zone (VZ), sub-ventricular zone (SVZ), and mantle zone (MZ). (B) Expression of sub-anatomical markers across sections from E12.5 (left), E13.5 2x coronal, 1x sagittal (middle), E14.5 (right). From left to right column: VZ markers, SVZ markers, and MZ markers. From top to bottom row: telencephalon markers (VZ: Fabp7 , SVZ: St18 , MZ: Dcx ); dorsal telencephalon markers (VZ: Pax6 , SVZ: Eomes , MZ: Tbr1 ), and ventral telencephalon markers (VZ/SVZ: Ascl1, Olig2 , MZ: Nkx2-1 ). (C) Cell type classification following unsupervised clustering and manual annotation. WT sections (top row) and Dlx1/Dlx2 -/- sections (bottom row) for E12.5 (left), E13.5 (2x coronal, 1x sagittal) (middle) and E14.5 (right). v/dNP: ventral/dorsal neural progenitors, v/dIP: ventral/dorsal intermediate progenitors, ThalNeur: thalamic neuron, HypothalNeur: hypothalamic neuron. (D) UMAP plots showing clustering and cell type classification for WT and Dlx1/Dlx2 -/- sections. Legend as shown in (C). (E) Dot plot showing the mean expression of cluster markers in cells in each cluster from (C). Dot sizes denote the fraction of cells expressing the corresponding markers. (F) Bar plot showing the percentage of each cell type in WT and Dlx1/Dlx2 -/- E12.5-E14.5 spatial <t>transcriptomics</t> dataset, summarised based on age and genotype. Legend in (C). (G) Volcano plot showing the differential expression analyses comparing VZ and SVZ of WT vs Dlx1/Dlx2 -/- GE. Thresholds for differentially expressed genes were set at FDR<0.05 and fold change > 1 or < -1. (H) Gene ontology analysis results of all differentially expressed genes in VZ and SVZ of Dlx1/Dlx2 -/- GE.
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    (A) Schematic diagram illustrating the sub-anatomical compartments within the embryonic telencephalon, including the ventricular zone (VZ), sub-ventricular zone (SVZ), and mantle zone (MZ). (B) Expression of sub-anatomical markers across sections from E12.5 (left), E13.5 2x coronal, 1x sagittal (middle), E14.5 (right). From left to right column: VZ markers, SVZ markers, and MZ markers. From top to bottom row: telencephalon markers (VZ: Fabp7 , SVZ: St18 , MZ: Dcx ); dorsal telencephalon markers (VZ: Pax6 , SVZ: Eomes , MZ: Tbr1 ), and ventral telencephalon markers (VZ/SVZ: Ascl1, Olig2 , MZ: Nkx2-1 ). (C) Cell type classification following unsupervised clustering and manual annotation. WT sections (top row) and Dlx1/Dlx2 -/- sections (bottom row) for E12.5 (left), E13.5 (2x coronal, 1x sagittal) (middle) and E14.5 (right). v/dNP: ventral/dorsal neural progenitors, v/dIP: ventral/dorsal intermediate progenitors, ThalNeur: thalamic neuron, HypothalNeur: hypothalamic neuron. (D) UMAP plots showing clustering and cell type classification for WT and Dlx1/Dlx2 -/- sections. Legend as shown in (C). (E) Dot plot showing the mean expression of cluster markers in cells in each cluster from (C). Dot sizes denote the fraction of cells expressing the corresponding markers. (F) Bar plot showing the percentage of each cell type in WT and Dlx1/Dlx2 -/- E12.5-E14.5 spatial <t>transcriptomics</t> dataset, summarised based on age and genotype. Legend in (C). (G) Volcano plot showing the differential expression analyses comparing VZ and SVZ of WT vs Dlx1/Dlx2 -/- GE. Thresholds for differentially expressed genes were set at FDR<0.05 and fold change > 1 or < -1. (H) Gene ontology analysis results of all differentially expressed genes in VZ and SVZ of Dlx1/Dlx2 -/- GE.
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    (A) Schematic diagram illustrating the sub-anatomical compartments within the embryonic telencephalon, including the ventricular zone (VZ), sub-ventricular zone (SVZ), and mantle zone (MZ). (B) Expression of sub-anatomical markers across sections from E12.5 (left), E13.5 2x coronal, 1x sagittal (middle), E14.5 (right). From left to right column: VZ markers, SVZ markers, and MZ markers. From top to bottom row: telencephalon markers (VZ: Fabp7 , SVZ: St18 , MZ: Dcx ); dorsal telencephalon markers (VZ: Pax6 , SVZ: Eomes , MZ: Tbr1 ), and ventral telencephalon markers (VZ/SVZ: Ascl1, Olig2 , MZ: Nkx2-1 ). (C) Cell type classification following unsupervised clustering and manual annotation. WT sections (top row) and Dlx1/Dlx2 -/- sections (bottom row) for E12.5 (left), E13.5 (2x coronal, 1x sagittal) (middle) and E14.5 (right). v/dNP: ventral/dorsal neural progenitors, v/dIP: ventral/dorsal intermediate progenitors, ThalNeur: thalamic neuron, HypothalNeur: hypothalamic neuron. (D) UMAP plots showing clustering and cell type classification for WT and Dlx1/Dlx2 -/- sections. Legend as shown in (C). (E) Dot plot showing the mean expression of cluster markers in cells in each cluster from (C). Dot sizes denote the fraction of cells expressing the corresponding markers. (F) Bar plot showing the percentage of each cell type in WT and Dlx1/Dlx2 -/- E12.5-E14.5 spatial transcriptomics dataset, summarised based on age and genotype. Legend in (C). (G) Volcano plot showing the differential expression analyses comparing VZ and SVZ of WT vs Dlx1/Dlx2 -/- GE. Thresholds for differentially expressed genes were set at FDR<0.05 and fold change > 1 or < -1. (H) Gene ontology analysis results of all differentially expressed genes in VZ and SVZ of Dlx1/Dlx2 -/- GE.

    Journal: bioRxiv

    Article Title: The DLX/Notch axis is necessary for spatiotemporal regulation of neural cell fate

    doi: 10.1101/2025.09.28.679022

    Figure Lengend Snippet: (A) Schematic diagram illustrating the sub-anatomical compartments within the embryonic telencephalon, including the ventricular zone (VZ), sub-ventricular zone (SVZ), and mantle zone (MZ). (B) Expression of sub-anatomical markers across sections from E12.5 (left), E13.5 2x coronal, 1x sagittal (middle), E14.5 (right). From left to right column: VZ markers, SVZ markers, and MZ markers. From top to bottom row: telencephalon markers (VZ: Fabp7 , SVZ: St18 , MZ: Dcx ); dorsal telencephalon markers (VZ: Pax6 , SVZ: Eomes , MZ: Tbr1 ), and ventral telencephalon markers (VZ/SVZ: Ascl1, Olig2 , MZ: Nkx2-1 ). (C) Cell type classification following unsupervised clustering and manual annotation. WT sections (top row) and Dlx1/Dlx2 -/- sections (bottom row) for E12.5 (left), E13.5 (2x coronal, 1x sagittal) (middle) and E14.5 (right). v/dNP: ventral/dorsal neural progenitors, v/dIP: ventral/dorsal intermediate progenitors, ThalNeur: thalamic neuron, HypothalNeur: hypothalamic neuron. (D) UMAP plots showing clustering and cell type classification for WT and Dlx1/Dlx2 -/- sections. Legend as shown in (C). (E) Dot plot showing the mean expression of cluster markers in cells in each cluster from (C). Dot sizes denote the fraction of cells expressing the corresponding markers. (F) Bar plot showing the percentage of each cell type in WT and Dlx1/Dlx2 -/- E12.5-E14.5 spatial transcriptomics dataset, summarised based on age and genotype. Legend in (C). (G) Volcano plot showing the differential expression analyses comparing VZ and SVZ of WT vs Dlx1/Dlx2 -/- GE. Thresholds for differentially expressed genes were set at FDR<0.05 and fold change > 1 or < -1. (H) Gene ontology analysis results of all differentially expressed genes in VZ and SVZ of Dlx1/Dlx2 -/- GE.

    Article Snippet: Samples were prepared and processed in accordance with manufacturer’s instructions (BGI STOmics Stereo-seq Transcriptomics set for Chip-on-a-slide user manual version B) using the STOmics Transcriptomics kit (STOmics Cat# 111KT114).

    Techniques: Expressing, Quantitative Proteomics